STK23 (SRPK3) (NM_014370) Human Recombinant Protein PH35881M5 from Leading Biology

Product Specs
ItemSTK23 (SRPK3) (NM_014370) Human Recombinant Protein
CompanyLeading Biology
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Catalog NumberPH35881M5
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
Quantity20 ug
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene NameSRSF protein kinase 3
NCBI Gene AliasesMSSK-1, STK23, MSSK1
Molecule NameSRPK3
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight61.8 kDa
SpeciesHuman
ActivitySRPK3 activity verified in a biochemical assay: SRPK3 (SRSF protein kinase 3) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. SRPK3 is a serine/threonine kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. Varying concentrations of SRPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors